Automated Massively Parallel Sequencing Library & Sample Preparation in Forensics
Overall Workflow
Next-generation sequencing (NGS) technology is transforming Forensic Genetic Genealogy (FGG), enabling investigators to rapidly analyze large volumes of DNA data in criminal investigations and cases involving unidentified individuals. The VERSA1100 is a validated liquid handler compatible with Qiagen/Verogen’s ForenSeq Kits, including the ForenSeq® Kintelligence Kit, ForenSeq® mtDNA Whole Genome Kit, and ForenSeq® DNA Signature Prep Kit. Internal validation studies have demonstrated the effectiveness of VERSA 1100 NGS system in analyzing DNA inputs ranging from 8 pg to 4 ng, making it suitable for sequencing highly degraded forensic samples.
VERSA Automated Forensic NGS Library Preparation Applications
- DNA/RNA Purification and Cleanup
- DNA/RNA Fragment Size Selection
- Enzymatic Reaction Setup
- Library Normalization and Pooling
- Single and Multiplex RT-PCR Setup
- Sequencing Reaction Setup
- Sexual Assault Kits Processing
- Oligo-based Gene Synthesis Setup
- Magnetic Bead Based Applications
Specification | VERSA 1100 MPS |
| Deck Capacity | 15 |
| Pipetting Head (Single Channel Function, Disposable Tip) | 8 or 96 channel or 4 channel with independent Z-axis |
| ReagentDrop | 8 channels |
| Magnetic Block | 1 |
| Temperature Regulation Block | 2 |
| Reagent Cooling Block | 1 |
| Plate Shaker | 1 |
| Gripper | 1 |
| 96-Tip Aspirator | Optional |
| Liquid Level Sensing | Optional |
| HEPA/UV/LED Enclosure | Included |
| Width x Depth x Height (cm) | 98.5 x 75.2 x 108.2 |
| Weight (kg) | 200 |
Independently Validated
Automating ForenSeq Kits Using Aurora Biomed’s VERSA 1100 Liquid Handling Workstation.
This application note/webinar shows three separate internal validation studies leveraging Aurora Biomed’s VERSA 1100 automation platform and the industry-leading forensic NGS library preparation chemistry – the ForenSeq® workflow by Verogen Inc.
The Dive into Next Generation Sequencing: From Validation to Implementation and Beyond!
This poster reviews how three different platforms of NGS, including Verogen ForenSeq DNA Signature Prep Primer Set B Kit, ForenSeq mtDNA Whole Genome Kit, and ForenSeq Kintelligence Kit, were validated in accordance with the FBI Quality Assurance Standards (QAS) by using VERSA 1100.
Modules Tailored for Automated NGS Library Preparation Protocols
Magnetic Bead Vortex
ensures homogeneous bead suspension
96 Tip Aspirator
increases efficiency and speed while reducing tip costs
Shaker-Heater
allows efficient sample processing
Reagent Block Cooler
preserves precious dNTPs, buffers and enzymes
ReagentDrop
draws reagents from their reservoir and eliminates tip usage
Gripper
moves sample plates between different deck positions
Magnetic Block
pulls down magnetic beads for NGS automation
Magnetic bead mixer
ensures homogeneous bead suspension
- Open System – compatible with commercially available kits including Illumina, Thermo Fisher, etc…
- HEPA/UV/LED Enclosure – keeps samples contamination-free
- Scalable Solution – processes 1 to 96 samples in parallel depending on throughput demands
- Unparalleled Customer Service – our engineering team is readily available to troubleshoot your protocols
How user-friendly is VERSA software for NGS automation?
The VERSA software is exceptionally user-friendly for NGS automation. It simplifies the process of programming, executing, and monitoring automated NGS workflows, making it highly intuitive for users.
Can VERSA handle high-throughput NGS applications?
Yes, VERSA is equipped to handle high-throughput NGS workflows, accommodating from a minimum of one sample up to 96 samples in a single run, depending on the specific workflow and configuration.
How many samples can VERSA process simultaneously in a high-throughput setting?
VERSA is designed to handle a scalable number of samples simultaneously, accommodating from minimum to 96 samples in a single run, depending on the specific workflow and configuration.
Is it possible to use solely the heater of the heater/Shaker module?
Certainly! While conducting various protocols using the Heater-Shaker Module, it might be necessary for the module to provide heat without the shaking function. The Heater-Shaker Module offers this capability.
What kits can be used with the VERSA automated NGS library and sample preparation system?
Our VERSA automated workstations are very flexible and open systems compatible with almost all the Next Generation Sequencing (NGS) library and sample preparation kits, reagents and labware in the market.
Can VERSA be used to incubate samples on deck?
Yes, the VERSA Automated Nucleic Acid Extraction and Purification workstation is equipped with a Heater-Cooler Plate and Heater-Shaker that can be used for incubating samples at temperatures from 2 – 92°C.
Which steps of NGS library prep can be automated on VERSA?
All the steps from DNA/RNA purification and cleanup, DNA/RNA fragment size selection, enzymatic reaction setup, library construction, normalization and pooling, single and multiplex real-time PCR setup, and sequencing reaction setup can be automated on VERSA. Transferring the plate to a thermocycler for PCR reaction and centrifuging steps should be done manually.
Can user set up a pause point to take plates for centrifugation and etc.?
Yes, when manual intervention is required, a pause message can be defined to pop up on the monitor to notify the user about the next manual steps. VERSA Automated NGS library preparation system can also email the pause notifications to users who should take responsibility for the step.
Does ReagentDrop cause any splashing?
No, the ReagentDrop dispensing speed on the VERSA automated NGS library and sample preparation workstation is always optimized for reagents with different consistency to avoid splashing.
Is there any chance of Cross-contamination?
No, we have validated the VERSA for automating different applications and have not observed any cross-contamination issues. The VERSA automated NGS library preparation workstation utilizes disposable tips to eliminate cross-contamination. Additionally, the aspirating and dispensing speed is carefully adjusted to avoid splashing or carry-over contamination. VERSA is also equipped with a HEPA filter and UV light to keep the environment contamination-free.
Does VERSA automated NGS library prep workstation only work with specific sequencers?
VERSA is a very flexible and open system and is capable of preparing libraries/samples for any NGS instrument.