PRODUCT INFORMATION
ADNap 20 is a fully automated benchtop system for the high-throughput extraction of nucleic acids from a variety of sources. The system relies on an automated 96-channel magnetic head for reliable and rapid extraction of high-quality nucleic acids. Like all of Aurora’s liquid handlers, this system is open and flexible. 96-channel magnetic head 32-channel magnetic head 16-channel magnetic head Extracted nucleic acids can be used for a number of applications including The ADNap 20 uses a 96-channel head of magnetic bars combined with disposable tip combs to mix and transfer magnetic beads. The magnetic bars move up and down, allowing mixing of the reagents and beads following lysis. Once the magnetic beads collect at the bottom of the tip comb (that surrounds the magnetic rods) the magnetic head moves to a different plate and the beads are released into the new plate by moving the magnetic rods out of the tip comb. This process is repeated for bead washing and elution steps. Positive COVID-19 samples (n=20) were extracted by three different methods (reagents), and the positivity detection rates were compared. Saliva samples were diluted with normal saline at 1:9 dilution and made up to 50 and 30 copies/mL of the viral culture. Gel electrophoresis and nanodrop readings of DNA extracted from whole blood. Agarose gel electrophoresis of gDNA samples from blood shows a high yield of genomic DNA using ADNAP-20. The bands are clear without smearing indicating pure and undegraded gDNA. Nanodrop readings of DNA extracted from whole blood. The readings are around the 1.8 value indicating pure gDNA. ADNap 20 is a fully automated benchtop system for the high-throughput extraction of nucleic acids from a variety of sources. The system relies on an automated 96-channel magnetic head for reliable and rapid extraction of high-quality nucleic acids. We recommend that you use plates from Aurora Biomed since they were specifically designed to be used with ADNap 20-tip combs. Plates from other manufacturers may not be a complete fit with the tip combs and can cause cross-contamination issues. The instrument will not start the protocol without the tip combs inserted into the tip comb holder. We strongly recommend that volumes are within defined limits to avoid spillover and for the magnetic processor to work efficiently. Since deep well plates 96-well PCR plates can be used during the same run, it is possible to process large volumes (in a deep well plate) and elute the purified nucleic acids into a smaller volume in a PCR plate. Most commercially available magnetic beads >100 nm in size will work with the ADNap 20. The main consumables needed are – 96-channel deep well plates and 96-channel deep well tip combs – both of which can be purchased from Aurora Biomed. You will also need 96-channel standard PCR plates. ADNap 20 has a deck of 6 slots.
The ADNap Series
What does ADNap 20 system do?
What plates can be used with the ADNap 20?
What happens if I forget to insert the tip combs into the tip comb holder?
Are the volumes of reagents in each well critical?
Can I concentrate the sample during the run?
Can I use any magnetic bead-based nucleic acid extraction kit?
What consumables do I need for carrying out magnetic bead-based extraction using ADNAP 20?
How many slots does the instrument have?
